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Total protein measurement using a fiber-optic evanescent wave-based biosensor
| Content Provider | Semantic Scholar |
|---|---|
| Author | Preejith, P. V. Lim, Chae Sun Kishen, Anil John, M. Shelly Asundi, Anaud |
| Copyright Year | 2004 |
| Abstract | A novel method and instrumental system to determine the total protein concentration in a liquid sample is described. It uses a fiber optic total protein sensor (FOPS) based on the principles of fiber optic evanescent wave spectroscopy. The FOPS applies a dye-immobilized porous glass coating on a multi-mode optical fiber. The evanescent waves at the fiber optic core-cladding interface are used to monitor the protein-induced changes in the sensor element. The FOPS offers a single-step method for quantifying protein concentrations without destroying the sample. The response time and reusability of the FOPS are evaluated. This unique sensing method presents a sensitive and accurate platform for the quantification of protein. |
| Starting Page | 105 |
| Ending Page | 110 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| DOI | 10.1023/A:1021955032291 |
| Alternate Webpage(s) | http://bios18.tripod.com/gfp/protein.pdf |
| PubMed reference number | 12882283 |
| Alternate Webpage(s) | https://doi.org/10.1023/A%3A1021955032291 |
| Journal | Medline |
| Volume Number | 25 |
| Journal | Biotechnology Letters |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |