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Oblique-incidence reflectivity difference microscope for label-free high-throughput detection of biochemical reactions in a microarray format.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhu, Xiangdong Landry, James P. Sun, Yung-Shin Gregg, Jeff P. Guo, Xiaowen |
| Copyright Year | 2007 |
| Abstract | We describe a recently developed oblique-incidence reflectivity difference (OI-RD) microscope, a form of polarization-modulated imaging ellipsometer, for label-free-high-throughput detection of biomolecular reactions on DNA and protein microarrays. We present examples of application of this technique to end-point and real-time investigations of DNA-DNA hybridization, antibody-antigen capture, and protein-small-molecule binding reactions. Compared to a conventional imaging ellipsometer based on the polarizer-compensator-sample-analyzer scheme and under the off-null condition, a polarization-modulated OI-RD microscope is inherently more sensitive by at least 1 order of magnitude to thickness changes on a solid surface. Compared with imaging surface plasmon resonance microscopes based on reflectance change on falling or rising slopes of the surface plasmon resonance, the OI-RD microscope (1) has a comparable sensitivity, (2) is applicable to conventional microscope glass slides, and (3) easily covers a field of view as large as the entire surface of a 1 in. x 3 in. (2.54 cm x 7.62 cm) microscope slide. |
| File Format | PDF HTM / HTML |
| DOI | 10.1364/BIO.2006.TuC8 |
| PubMed reference number | 17356635 |
| Journal | Medline |
| Volume Number | 46 |
| Issue Number | 10 |
| Alternate Webpage(s) | http://zhu.physics.ucdavis.edu/publication%20in%20pdf/AppliedOptics_2007_Feature_Zhu.pdf |
| Alternate Webpage(s) | https://doi.org/10.1364/BIO.2006.TuC8 |
| Journal | Applied optics |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
