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Hepatic knockdown of stearoyl-CoA desaturase 1 via RNA interference in obese mice decreases lipid content and changes fatty acid composition.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Xu, Haiyan Wilcox, Denise Nguyen, Phong Van Brodjian, Sevan J. Suhar, Thomas Collins, Christine A. Landschulz, Katherine T. |
| Copyright Year | 2007 |
| Abstract | Stearoyl-CoA desaturases (SCDs) catalyze the biosynthesis of monounsaturated fatty acids from saturated fatty acids. Four scd genes have been identified in mice and three in human (including one pseudogene). Among the four mouse SCD isoforms, SCD1 is predominantly expressed in liver and adipose tissue. Mice null for the scd1 gene have reduced adiposity, increased energy expenditure and altered lipid profiles. To further evaluate the specific role of hepatic SCD1 and the potential to achieve similar desirable phenotypic changes in adult obese mice, adenovirus-mediated short hairpin interfering RNA (shRNA) was used to acutely knock down hepatic scd1 expression in ob/ob mice. Robust reductions in hepatic SCD1 mRNA and SCD1 enzymatic activity were achieved, sustained up to 2 weeks. Reduced hepatic content of neutral lipids and robust lowering of lipid desaturation indexes, but increased content of liver phosphotidylcholine were observed with SCD1 knockdown. Increased total plasma cholesterol levels were also observed. No significant changes in body weight were observed. Expression levels of several lipogenic and lipid oxidation genes were not significantly altered by short term SCD1 reduction, but UCP2 expression was increased. Our results demonstrate that significant changes to both hepatic and systemic lipid profiles can be achieved through specific knockdown of liver-expressed SCD1 in the ob/ob mouse model. However, hepatic SCD1 knockdown does not result in significant changes in body weight in the short term. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.bioscience.org/fbs/getfile.php?FileName=/2007/v12/af/2352/2352.pdf |
| PubMed reference number | 17485339v1 |
| Volume Number | 12 |
| Journal | Frontiers in bioscience : a journal and virtual library |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Adipose tissue Anabolism Cholesterol Energy Metabolism Fatty Acids Fatty Acids, Monounsaturated Fatty Liver Fatty degeneration Index Liver diseases Null Value Obesity Palmitoyl-CoA Hydrolase Pseudogenes RNA Interference SCD gene SCD wt Allele Short Hairpin RNA Stearoyl-CoA Desaturase lipid oxidation ob/ob Mouse |
| Content Type | Text |
| Resource Type | Article |
