Effect of alcohols on gastric and small intestinal apical membrane integrity and fluidity.

Content Provider	Semantic Scholar
Author	Ballard, Heather J. Wilkes, James Hirst, Barry Hugo
Copyright Year	1988
Abstract	Duodenal and jejunal brush border membrane vesicle integrity was studied after in vitro treatment of rabbit tissue with ethyl, benzyl or octyl alcohol. The effects of the alcohols on gastric parietal cell apical and microsomal membrane vesicle integrity was also studied. Membrane vesicle integrity was determined from the enclosed volume of the vesicle preparations, measured as [14C]glucose space at equilibrium. Exposure of vesicles to the three alcohols caused concentration dependent decreases in enclosed volume. The rank order of potency of the alcohol was octyl greater than benzyl greater than ethyl. Concentrations greater than or equal to 10 mM benzyl alcohol significantly reduced the enclosed volume of duodenal or jejunal vesicles; jejunal vesicles were disrupted by 625 mM ethanol, whereas 2 M ethanol was required to disrupt the duodenal vesicles. Gastric apical membrane integrity was reduced with 0.25 M ethanol, the vesicles being approximately an order of magnitude more sensitive to ethanol than gross estimates of gastric mucosal damage, but 1 M ethanol was required to significantly damage gastric microsomes. All concentrations of benzyl or octyl alcohol tested (greater than or equal to 5 mM) reduced the enclosed volume of both gastric apical membrane vesicles and gastric microsomes. As determined by shrink-swell techniques, benzyl alcohol permeated duodenal vesicles at a faster rate than NH4Cl (apparent rate constant of 9.89 (0.71) X 10(-3)s-1 compared with 4.48 (0.23) X 10(-3)s-1). Therefore, reductions in enclosed volume in response to alcohol treatment could not be explained by alcohol induced osmotic shrinkage. The enclosed volume of the vesicles after alcohol treatment was negatively correlated with membrane fluidity suggesting a common causal effect, the increased fluidity increasing membrane fragility. Duodenal vesicles were more resistant to disruption by the alcohols compared with gastric and jejunal vesicles.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://gut.bmj.com/content/gutjnl/29/12/1648.full.pdf
PubMed reference number	3220304v1
Volume Number	29
Issue Number	12
Journal	Gut
Language	English
Access Restriction	Open
Subject Keyword	Alcohols Benzyl Alcohol Duodenum Estimated Ethanol Gastric mucosa Jejunum Microsomes Mucous Membrane Parietal Cells, Gastric Tissue membrane Vesicle (morphologic abnormality) apical membrane brush border membrane octyl alcohol response to alcohol viral capsid secondary envelopment
Content Type	Text
Resource Type	Article