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The acute regulation of glucose absorption, transport and metabolism in rat small intestine by insulin in vivo.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Jamal, Ammarah Wollen, N. |
| Copyright Year | 1984 |
| Abstract | The effect of acute changes in insulin concentrations in vivo on the absorption, transport and metabolism of glucose by rat small intestine in vitro was investigated. Within 2 min of the injection of normal anaesthetized rats with anti-insulin serum, lactate production and glucose metabolism were respectively diminished to 28% and 21% of normal and the conversion of glucose into lactate became quantitative. These changes correlated with the inhibition of two mucosal enzymes, namely the insulin-sensitive enzyme pyruvate dehydrogenase, and phosphofructokinase, which was shown by cross-over measurements to be the rate-limiting enzyme of glycolysis in mucosa. The proportion of glucose translocated unchanged from the luminal perfusate to the serosal medium was simultaneously increased from 45% to 80%. All the changes produced by insulin deficiency were completely reversed with 2 min when antiserum was neutralized by injection of insulin in vivo. The absorption and transport of 3-O-methylglucose were unaffected by insulin. It is concluded that glucose metabolism in rat small intestine is subject to short-term regulation by insulin in vivo and that glucose absorption and transport are regulated indirectly in response to changes in metabolism. Moreover, transport and metabolism compensate in such a way as to deliver the maximal 'effective' amount of glucose to the blood, whether as glucose itself or as lactate for hepatic gluconeogenesis. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.biochemj.org/content/ppbiochemj/219/3/1027.full.pdf |
| PubMed reference number | 6234885v1 |
| Volume Number | 219 |
| Issue Number | 3 |
| Journal | The Biochemical journal |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Eighty Gluconeogenesis Glycolysis In Vitro [Publication Type] Lactic acid Mucous Membrane PHOSPHOFRUCTOKINASE Phenobarbital Pyruvates glucose metabolism pyruvate dehydrogenase |
| Content Type | Text |
| Resource Type | Article |
