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The actin-based nanomachine at the leading edge of migrating cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Abraham, Violet Krishnamurthi, Vidhya Taylor, D. Lansing Lanni, Frederick |
| Copyright Year | 1999 |
| Abstract | Two fundamental parameters of the highly dynamic, ultrathin lamellipodia of migrating fibroblasts have been determined-its thickness in living cells (176 +/- 14 nm), by standing-wave fluorescence microscopy, and its F-actin density (1580 +/- 613 microm of F-actin/microm(3)), via image-based photometry. In combination with data from previous studies, we have computed the density of growing actin filament ends at the lamellipodium margin (241 +/- 100/microm) and the maximum force (1.86 +/- 0.83 nN/microm) and pressure (10.5 +/- 4.8 kPa) obtainable via actin assembly. We have used cell deformability measurements (. J. Cell Sci. 44:187-200;. Proc. Natl. Acad. Sci. USA. 79:5327-5331) and an estimate of the force required to stall the polymerization of a single filament (. Proc. Natl. Acad. Sci. USA. 78:5613-5617;. Biophys. J. 65:316-324) to argue that actin assembly alone could drive lamellipodial extension directly. |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/S0006-3495(99)77018-9 |
| PubMed reference number | 10465781 |
| Journal | Medline |
| Volume Number | 77 |
| Issue Number | 3 |
| Alternate Webpage(s) | http://www.rpgroup.caltech.edu/courses/aph161/Handouts/Abraham1999.pdf |
| Alternate Webpage(s) | https://doi.org/10.1016/S0006-3495%2899%2977018-9 |
| Journal | Biophysical journal |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
