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Development of glycosylated human interleukin-1α, neoglyco IL-1 alpha, by coupling with D-galactose monosaccharide: synthesis and purification
| Content Provider | Semantic Scholar |
|---|---|
| Author | Chiba, Taku Nabeshima, Sachi Takei, Yutaka Onozaki, Kikuo |
| Copyright Year | 2004 |
| Abstract | In order to develop glycosylated cytokine, recombinant human IL-1 alpha was chemically modified with galactose monosaccharide. Galactose with C9 spacer, 8-(hydrazinocarbonyl)octyl beta-D-galctopyranoside (3), was synthesized by glycosylation of C9 spacer, methyl 9-hydroxynonanoate, with acetobromogalactose, followed by deacetylation and hydrazidation. Total yield of 3 was 43.6% in three steps. Compound 3 was coupled to IL-1 alpha by the acyl azide method. The glycosylated IL-1 was purified by anion-exchange chromatography, and galactose coupled to IL-1 was confirmed by R. communis lectin blotting. Based on the molecular weight, the average number of carbohydrate molecules introduced per molecule of IL-1 alpha was estimated to be 9.1. |
| Starting Page | 63 |
| Ending Page | 67 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| DOI | 10.1023/A:1006991416735 |
| PubMed reference number | 9530957 |
| Journal | Medline |
| Volume Number | 15 |
| Alternate Webpage(s) | https://page-one.springer.com/pdf/preview/10.1023/A:1006991416735 |
| Alternate Webpage(s) | https://doi.org/10.1023/A%3A1006991416735 |
| Journal | Glycoconjugate Journal |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
