NDLI: Characterization and Cryopreservation of Malus sieversii Seeds

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Characterization and Cryopreservation of Malus sieversii Seeds

Content Provider	Semantic Scholar
Author	Kushnarenko, Svetlana V. Sal’nikov, E. V. Nurtazin, Marat Mukhitdinova, Zinat Rakhimbaev, Izbasar Reed, Barbara M.
Copyright Year	2010
Abstract	Kazakhstan is one of the centers of origin and evolution of apple (Malus). The main forest species is Malus sieversii (Ledeb.) M. Roem., representatives of which have important traits such as disease and insect resistance, cold hardiness, and fruit quality. The area of these unique wild forests is continually decreasing as a result of human activities. An expedition was held in September 2008 to collect and safeguard the genetic resources of Malus sieversii in Kazakhstan. Over 4400 seeds from the fruit of 34 Malus sieversii accessions were collected in the Zailyiski Alatau Mountains near Almaty (Bel-Bulak and Issyk Gorges). The trees where fruit was collected showed high variability in habit and fruit size, shape and flavor. Fruit color varied from yellow white to red. Seeds were variable in both color and length. We tested seed cryopreservation for long-term storage of wild Malus biodiversity. Seeds were air dried to a mean moisture content of 8.23%. Seeds were directly immersed in liquid nitrogen (LN) for 30 min and rewarmed at room temperature to test the effect of LN exposure. Viability following LN as evaluated by 2,3,5-triphenyltetrazolium chloride (TZ) ranged from 72.4% to 90.0% and did not differ significantly from the controls or from the germination tests. Germination tests indicated slightly but not significantly higher viability for LN exposed seeds, but germination vigor for LN exposed seeds was significantly higher than controls over the 6 week germination period. These results indicate that cryopreservation in LN is an option for long-term conservation of this important germplasm. _____________________________________________________________________________________________________________
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://globalsciencebooks.info/Online/GSBOnline/images/2010/AAJPSB_4(SI1)/AAJPSB_4(SI1)5-9o.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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