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TRAIL expression in vascular smooth muscle.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Gochuico, Bernadette R. Zhang, Jin Ma, Bruce Yong Marshak-Rothstein, Ann Fine, Alan |
| Copyright Year | 2000 |
| Abstract | TRAIL is a cell-associated tumor necrosis factor-related apoptosis-inducing ligand originally identified in immune cells. The ligand has the capacity to induce apoptosis after binding to cell surface receptors. To examine TRAIL expression in murine vascular tissue, we employed in situ hybridization and immunohistochemistry. In these studies, we found that TRAIL mRNA and protein were specifically localized throughout the medial smooth muscle cell layer of the pulmonary artery. Notably, a similar pattern of expression was observed in the mouse aorta. Consistent with these findings, we found that cultures of primary human aorta and pulmonary artery smooth muscle cells express abundant TRAIL mRNA and protein. We also found that these cells and endothelial cells undergo cell lysis in response to exogenous addition of TRAIL. Last, we confirmed that TRAIL specifically activated a death program by confirming poly(ADP ribose) polymerase cleavage. Overall, we believe that these findings are relevant to understanding the factors that regulate cell turnover in the vessel wall. |
| Starting Page | 713 |
| Ending Page | 715 |
| Page Count | 3 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ajplung.physiology.org/content/ajplung/278/5/L1045.full.pdf |
| Alternate Webpage(s) | http://ajplung.physiology.org/content/ajplung/278/5/l1045.full.pdf |
| PubMed reference number | 10781437v1 |
| Volume Number | 278 |
| Issue Number | 5 |
| Journal | American journal of physiology. Lung cellular and molecular physiology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Aorta Apoptosis Artery Smooth Muscle Tissue Blood Vessel Tissue In Situ Hybridization Ligands Muscle Cells Muscle, Smooth, Vascular Myocytes, Smooth Muscle Nucleic Acid Hybridization Pulmonary artery structure Ribose Smooth muscle (tissue) pathologic cytolysis tumor necrosis |
| Content Type | Text |
| Resource Type | Article |
