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Pisatin metabolism in pea (Pisum sativum L.) cell suspension cultures
| Content Provider | Semantic Scholar |
|---|---|
| Author | Borejsza-Wysocki, Wlodzimierz Borejsza-Wysocka, Ewa Hrazdina, Géza |
| Copyright Year | 2005 |
| Abstract | ABSTRACTCell suspension cultures were established from germinating pea (Pisum sativum L.) seeds. This cell culture, which accumulated pisatin, consisted mostly of single cells containing a few cell aggregates. The cells responded to treatment with a yeast glucan preparation with transient accumulation of pisatin in both cells and culture media. Addition of pisatin to cell cultures resulted in increased synthesis of pisatin. Phenylalanine ammonia-lyase, chalcone synthase and isoflavone reductase activities were present in untreated cells. Upon treatment with an elicitor preparation the activities of the first two enzymes showed a rapid, transient increase up to 20 hours after treatment. Isoflavone reductase showed a major and minor peak at 16 and 36 h, respectively, after elicitor treatment. The time course of the enzyme activity and pisatin accumulation is consistent with an elicitor-mediated response. |
| Starting Page | 304 |
| Ending Page | 309 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/BF01088286 |
| PubMed reference number | 30727668 |
| Journal | Medline |
| Volume Number | 16 |
| Alternate Webpage(s) | https://page-one.springer.com/pdf/preview/10.1007/BF01088286 |
| Alternate Webpage(s) | https://doi.org/10.1007/BF01088286 |
| Journal | Plant Cell Reports |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
