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Sumoylation of heterogeneous nuclear ribonucleoproteins, zinc finger proteins, and nuclear pore complex proteins: a proteomic analysis.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Li, Tianwei Evdokimov, Evgenij A. Shen, Rong-Fong Chao, Chien-Chung Tekle, Ephrem Wang, Tao Stadtman, Earl R. Yang, David C. Chock, P. Boon |
| Copyright Year | 2004 |
| Abstract | SUMO, a small ubiquitin-related modifier, is known to covalently attach to a number of nuclear regulatory proteins such as p53, IkappaB, promyelocytic leukemia protein and c-Jun. The sumoylation reaction is catalyzed by the SUMO protease, which exposes the C-terminal active glycine residue of the nascent SUMO, the heterodimeric SUMO activating enzyme, the SUMO conjugating enzyme, Ubc9, and SUMO protein ligases, in a manner similar to ubiquitinylation. Identification of SUMO-regulated proteins is hampered by the fact that many sumoylated proteins are present at a level below normal detection limit. This limitation was overcome by either in vivo overexpression of Myc-SUMO or in vitro sumoylation with excess biotin-SUMO and Ubc9. Sumoylated proteins so obtained were affinity purified or isolated by immunoprecipitation. The isolated sumoylated proteins were identified by sequence analysis using mass spectrometric methods. Results reveal that several heterogeneous nuclear ribonucleoproteins (hnRNPs), zinc finger proteins, and nuclear pore complex proteins were sumoylated. The sumoylation of hnRNP A1, hnRNP F, and hnRNP K were confirmed in vivo by coimmunoprecipitation. In view of the facts that hnRNPs have been implicated in RNA splicing, transport, stability, and translation, our findings suggest that sumoylation could play an important role in regulating mRNA metabolism. |
| Starting Page | 1 |
| Ending Page | 6 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.dtic.mil/dtic/tr/fulltext/u2/a449115.pdf |
| Alternate Webpage(s) | http://www.dtic.mil/get-tr-doc/pdf?AD=ADA449115 |
| Alternate Webpage(s) | https://dir.nhlbi.nih.gov/labs/lb/docs/Boon_Sumo_PNAS.pdf |
| PubMed reference number | 15161980v1 |
| Volume Number | 101 |
| Issue Number | 23 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Chemical Modifier Endopeptidases Genetic Heterogeneity Glycine Heterogeneous-Nuclear Ribonucleoprotein K Heterogeneous-Nuclear Ribonucleoproteins I-kappa B Proteins Immunoprecipitation Leukemia, B-Cell Ligase Nuclear Pore Complex Proteins Promyelocytic leukemia protein Proto-Oncogene Proteins c-myc RNA Splicing Sequence Analysis Sumoylation UBE2I gene Ubiquitination Zinc Fingers hnRNP A1 |
| Content Type | Text |
| Resource Type | Article |
