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True optical resolution beyond the Rayleigh limit achieved by standing wave illumination.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Frohn, Joachim Knapp, Helmut F. Stemmer, Andreas |
| Copyright Year | 2000 |
| Abstract | During the last decade, various efforts have been undertaken to enhance the resolution of optical microscopes, mostly because of their importance in biological sciences. Herein, we describe a method to increase the resolution of fluorescence microscopy by illuminating the specimen with a mesh-like interference pattern of a laser source and electronic postprocessing of the images. We achieve 100-nm optical resolution, an improvement by a factor of more than 2 compared with standard fluorescence microscopy and of 1.5 compared with confocal scanning. |
| File Format | PDF HTM / HTML |
| DOI | 10.1073/pnas.130181797 |
| PubMed reference number | 10840057 |
| Journal | Medline |
| Volume Number | 97 |
| Issue Number | 13 |
| Alternate Webpage(s) | http://www.pnas.org/content/97/13/7232.full.pdf |
| Alternate Webpage(s) | https://doi.org/10.1073/pnas.130181797 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |