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Expansion of Cd 34 + Human Hematopoietic Cells from Umbilical Cord Blood Using Roller
| Content Provider | Semantic Scholar |
|---|---|
| Author | Andrade-Zaldívar, Hera Kalixto-Sanchez, Marco A. Rosa, Ana Paulina Barba León-Rodríguez, Antonio De |
| Copyright Year | 2014 |
| Abstract | Hematopoietic stem cell (HSC) transplantation is limited by the initial CD34+ cell content in cord blood units; the aim of this work was the in vitro expansion of HSC to overcome this issue. Supplemented IMDM roller bottle cultures of CD34+ cells from human umbilical cord blood were established for hematopoietic progenitor expansion. The maximum total colony forming cells (CFC) expansion was achieved after 5 days of culture, being 16.94±2.82 folds in roller bottles and 17.28±4.47 in static cultures. However, the maximum total cell fold expansion was attained after 10 days of culture. It was of 20.31±6.18 for the roller bottles and 26.45±9.89 for the static cultures. The efficacy of the roller bottles system for short-term cultures of CD34+ cells and expanding hematopoietic progenitors in IMDM was demonstrated; encouraging the use of this culture system for other experiments and may be used for future clinical applications. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.scielo.org.mx/pdf/rmiq/v13n2/v13n2a4.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
