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Molecular analysis of human T-cell lymphotropic virus type 1 and 2 (HTLV-1/2) seroindeterminate blood donors from Northeast of Iran; evidence of proviral tax, env, and gag sequences
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zanjani, Delaram Sayadpour Shahabi, Majid |
| Copyright Year | 2011 |
| Abstract | Human T cell lymphotropic virus type 1 and 2 (HTLV-1/2) Western blot indeterminate results are a problem for blood banks in endemic areas. To determine the prevalence of HTLV-1/2 infection among indeterminate donors, we analyzed 130 cases from Mashhad, an HTLV-1/2 endemic area in Northeast Iran. The most frequent Western blot bands were GD21 alone (37.2%) followed by rgp46-2 alone (32.1%). We further tested 40 available DNA samples of these cases by PCR for viral sequences, tax, gag, and pol, and found five cases (12.5%) to be positive for two or three HTLV-1 genes. There were no significant age, sex, and blood group differences between PCR-positive and PCR-negative cases. Among PCR-positive individuals, the most prevalent Western blot bands were variable combinations of rgp46-1, GD21, and gp21. The mean of the optical density (OD) of the enzyme-linked immunosorbent assay (ELISA) test was significantly higher in PCR-positive individuals. The frequency of the rgp46-1 band was also significantly higher in PCR-positive cases compared to PCR-negative ones. In conclusion, the majority of HTLV-indeterminate donors lack the HTLV provirus and therefore are not considered infected. However, in some cases with higher ODs in the ELISA test and seroreactivity to env proteins, rgp46-1 and GD21 in particular may be indicative of infection and need further evaluation by molecular methods. |
| Starting Page | A66 |
| Ending Page | A66 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ftp.ncbi.nlm.nih.gov/pub/pmc/15/43/1742-4690-8-S1-A66.PMC3112781.pdf |
| PubMed reference number | 20929385v1 |
| Alternate Webpage(s) | https://doi.org/10.1089/aid.2010.0017 |
| DOI | 10.1089/aid.2010.0017 |
| Journal | AIDS research and human retroviruses |
| Volume Number | 8 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Bands Bank (environment) Blood Banks Encephalomyelitis, Western Equine Enzyme-Linked Immunosorbent Assay HIV Infections Human T-lymphotropic virus 1 Molecular Analysis Molecular Genetic Technique Proviruses T-Lymphocyte Western Blot Western Blotting |
| Content Type | Text |
| Resource Type | Article |
