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The Role of α1 (Connexin-43) Gap Junction Expression in Adrenal Cortical Cell Function.

Content Provider	Semantic Scholar
Author	Oyoyo, Ugochi A. Shah, Uzma S. Murray, Sandra Akiko
Copyright Year	1997
Abstract	To investigate the relationship between adrenal cell function and gap junction expression, a bovine adrenal cell line (SBAC) was studied. Western blot and immunocytochemical techniques were used to demonstrate gap junction expression in SBAC cell populations. Cells expressedα 1 (connexin 43) gap junction protein at points of cell-to-cell contact. Gap junction number and size increased in populations treated with ACTH (40 mU/ml) or dibutyryl cAMP (DbcAMP, 1.0 mm). Treatment with either ACTH or DbcAMP increased steroid production and cAMP levels. SBAC cell number, however, decreased in ACTH- or DbcAMP-treated populations. The number of cells increased in cultures transfected with α1-antisense complementary DNA. Neither ACTH nor DbcAMP treatment decreased cell number or increased steroidogenesis in α1-antisense complementary DNA-transfected cell populations. However, cell populations in which gap junctions were inhibited retained the capacity to increase cAMP production in response to ACTH (40 mU/ml) treatme...
Starting Page	5385
Ending Page	5397
Page Count	13
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://watermark.silverchair.com/endo5385.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAkswggJHBgkqhkiG9w0BBwagggI4MIICNAIBADCCAi0GCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQM9spFy7Ea_Ixtg8-UAgEQgIIB_ukDGU9gPsv-fAMxzN9jN1Oa-iN0TgBVqitw3AmJcgub_h-IF89bodKnkQ0gFQe8y10xO0bqKyeTSCOLrDCKgmxN3DGUqeulqYEdIERd5v_HjM7QiImoSLCwBrP6DRraBxtgLsoEh3Q6nBUtXi8ToXoMwGcbEcv5d0e-H9qvUBTJ57R_he5BvUfKtd2nNk0Q6DgLk4G3Sm1PFg5y1GslL4EAtA9jIBM4MyCsBSakh16DhpmD_T_vJoD9OcNfuNaT0JE9zOpzWPxmMz3Qwgiemfoqz0v8DcKMm3b1m0TS62dhEpMExLoavC92xnrJKtwN3maeTuz-O_0DkHDK9HllJr0P_roemIez9dKw-8bwaCelo0PsJTe9Ki2-qXuHTsXt85qlRSRdialD26jlh1KG8eoUIacNFhetB3bTpSCRW4aNJCN1Es3DHS9EQJ8y3AFUJO4u-lQ2Nova7sNRXY6RBaprI5oR4q8yJP4QmoFCL6Y2WLtQizEyThfUajggC8UlZqGuI0FKvYTRhfZowWJN4ij_OjE_3jxWd3Y-1MEnd2qMu-tuhcbSxfvhCtOPQnwNzu7A5AQx6FhT7jjTenEnl1wu7lmEcWKhISSBTinNeqsLxRdSNX0yfpMMKdTUmbgVeu_0d_aN3YOg6rAQitDSI4Wz76Ofnt2f55TDj_b_Sw
PubMed reference number	28199534v1
Alternate Webpage(s)	https://doi.org/10.1210/endo.138.12.5617
DOI	10.1210/endo.138.12.5617
Journal	Endocrinology
Volume Number	138
Issue Number	12
Language	English
Access Restriction	Open
Subject Keyword	8-chloro-cyclic adenosine monophosphate Adrenal Glands Amino Acids Bucladesine Cattle Cell Communication Cell Count Cell physiology Connexins Corticotropin Freeze Fracturing GJA1 protein, human Gap Junctions Gland Herpes zoster disease Intercellular Communication Process Ions Microtomy Nucleotides Oligosaccharides Senile Plaques Steroid biosynthesis Steroids Western Blotting Zona Glomerulosa Zona Reticularis second messenger
Content Type	Text
Resource Type	Article

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