NDLI: Synthesis of oligonucleotide inhibitors of DNA (Cytosine-C5) methyltransferase containing 5-azacytosine residues at specific sites.

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Synthesis of oligonucleotide inhibitors of DNA (Cytosine-C5) methyltransferase containing 5-azacytosine residues at specific sites.

Content Provider	Semantic Scholar
Author	García, Ramón Güimil Brank, Adam S. Christman, Judith K. Marquez, Victor E. Eritja, Ramón
Copyright Year	2001
Abstract	The incorporation of 5-azacytosine residues into DNA causes potent inhibition of DNA (Cytosine-C5) methyltransferases. The synthesis of oligodeoxyribonucleotides incorporating single or multiple 5-aza-2'-deoxycytidine residues at precise sites was undertaken to generate an array of sequences containing the reactive 5-azacytosine base as specific target sites for enzymatic methylation. Preparation of these modified oligonucleotides requires the use of 2-(p-nitrophenyl)ethyloxycarbonyl (NPEOC) groups for the protection of exocyclic amino functions. These groups are removed under mild conditions, thus avoiding conventional protocols that are detrimental to the integrity of the 5-azacytosine ring.
Starting Page	63
Ending Page	71
Page Count	9
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://digital.csic.es/bitstream/10261/122143/1/antisense2001.pdf
PubMed reference number	11838638v1
Volume Number	11
Issue Number	6
Journal	Antisense & nucleic acid drug development
Language	English
Access Restriction	Open
Subject Keyword	5-azacytosine Amino Acid Metabolism, Inborn Errors Cytosine Methylation Methyltransferase Oligodeoxyribonucleotides Oligonucleotides Protocols documentation decitabine
Content Type	Text
Resource Type	Article

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