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Influence of High-Pressure Steam Pretreatment on the Structure of Rice Husk and Enzymatic Saccharification in a Two-Step System
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kazeem, Muinat Olanike Shah, Umi Kalsom Md Baharuddin, Azhari Samsu Rahman, N. A. |
| Copyright Year | 2017 |
| Abstract | This study aimed at developing an operational high-pressure steam pretreatment (HPSP) to effectively modify rice husk for enzymatic saccharification. The HPSP was performed at 160 to 200 °C under 0.3 to 2.8 MPa for 2 to 10 min. The efficiency of this method was based on the chemical composition, scanning electron microscopy (SEM), Fourier transform infrared (FTIR), and X-ray diffraction (XRD) analyses. Optimum pretreatment conditions (200 °C, 1.85 MPa for 7 min), enzyme concentration at 30 FPU/g and temperature at 60 °C for 48 h of continuous saccharification effectively produced sugar (21.1 g/L = 0.422 g/g dry substrate) at a saccharification degree of 53.87%. Conducting a secondstep enzymatic saccharification resulted in additional sugar production (7.9 g/L = 0.158 g/g substrate) and a 20.44% saccharification degree. In contrast, the two-step saccharification process (48 and 24 h) achieved optimal sugar yield of 0.581 g/g substrate and saccharification degree of 73.5%. Additionally, the process improved the yield of monomeric sugars of glucose (0.465 g/g), xylose (0.010 g/g), and cellobiose (0.063 g/g). Therefore, the combination of the high-pressure steam pretreatment with thermostable cellulase from Bacillus licheniformis 2D55 in a two-step enzymatic saccharification process is an economically viable method in rice husk bioprocessing for sugar production. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://bioresources.cnr.ncsu.edu/wp-content/uploads/2017/07/BioRes_12_3_6207_Kazeem_SBR_Influence_High_Pressure_Steam_Treatm_Struct_Rice_Husk_Enzym_11868.pdf |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | 3-Mercaptopropionic Acid Behavior CELLULASE Cellobiose Computed Tomography Scanning Systems Floating-point unit Glucose Gram per Gram Guilty Gear Xrd Maxima and minima Multistage interconnection networks Rh Factors Scanning Electron Microscopy Spectroscopy, Fourier Transform Infrared Spectroscopy, Near-Infrared Steam Sugar Sugars Xylose |
| Content Type | Text |
| Resource Type | Article |
