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Darstellung und Charakterisierung von Kofaktoren des Elektronentransfers in artifizieller und nativer Proteinumgebung
| Content Provider | Semantic Scholar |
|---|---|
| Author | Breitenstein, Christoph |
| Copyright Year | 2006 |
| Abstract | The protein Myoglobin was used as matix-molecule to produce monomeric porphyrins in solution. The protein complexes of differen zinc pheophorbides and zinc protoporphyrin have been studied by UV/Vis and NMR spectroscopy. The protein stability versus unfolding and NMR results Based on quantum mechanical calculations analysis of EPR- and ENDOR-spectra of the light excited triplet state yielded identification of α-protons and methyl β-protons have been identified for the first time. The data acquired agrees well with previously published data on native reaction centers. The terminal electron acceptors, [4Fe4S] centers FA and FB in Photosystem I (PSI), have been modelled by peptides with 16 amino acid length synthezied by SPPS Fmoc strategy. Both peptides incorporated a [4Fe4S] cluster in oxidation state 2 /1 as prooven by UV/Vis, EPR and Mossbauer spectroscopy. The redoxpotential for the one electron reduction was found to be -470 mV for both modell peptides. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://duepublico.uni-duisburg-essen.de/servlets/DerivateServlet/Derivate-5705/Breitenstein_Diss.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
