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Enhancer effect of fluorescein on the luminol-H2O2-horseradish peroxidase chemiluminescence: energy transfer process.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Díaz, Aurora Navas García, José Angel González Lovillo, J. |
| Copyright Year | 1997 |
| Abstract | The chemiluminescence of the luminol-H2O2-horseradish peroxidase system is increased by fluorescein. Fluorescein produces an enhancement of the luminol chemiluminescence similar to that of phenolphthalein, by an energy transfer process from luminol to fluorescein. The maximum intensity and the total chemiluminescence emission (between 380 and 580 nm) of luminol with fluorescein was more than three times greater than without fluorescein; however, the emission duration was shorter. The emission spectra in the presence of fluorescein had two maxima (425 and 535 nm) and the enhancement was dependent on pH and fluorescein concentration. A mechanism is proposed to explain these effects. |
| Starting Page | 199 |
| Ending Page | 205 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.biblioteca.uma.es/bbldoc/articulos/1671880x.pdf |
| PubMed reference number | 9481607v1 |
| Volume Number | 12 |
| Issue Number | 4 |
| Journal | Journal of bioluminescence and chemiluminescence |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Chemiluminescence Energy Transfer Enhancer of transcription Fluorescein Luminol Maximum Peroxides Phenolphthalein Transference (Psychology) |
| Content Type | Text |
| Resource Type | Article |
