NDLI: Separation of Photosynthetic Pigments by High-performance Liquid Chromatography: Comparison of Column Performance, Mobile Phase, and Temperature☆

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Separation of Photosynthetic Pigments by High-performance Liquid Chromatography: Comparison of Column Performance, Mobile Phase, and Temperature☆

Content Provider	Semantic Scholar
Author	Indriatmoko Shioi, Yuzo Brotosudarmo, Tatas Hardo Panintingjati Limantara, Leenawaty
Copyright Year	2015
Abstract	Abstract High-performance liquid chromatography (HPLC) has been commonly used as method of separating and identifying photosynthetic pigments such as chlorophylls and carotenoids because of such advantages as speed, high resolution and sensitivity. In this technique, high separation relies largely on the type of column material. This study compared the efficiency of five reverse-phase columns, C8, C18, C18 monolithic, π-NAP, and cholester, for separation of photosynthetic pigments at several fixed conditions of mobile phase and temperature. This investigation also analysed the parameters of ΔtR and tR ratio for selected pigments and resolution for structural isomers, such as α- and β-carotene. Among above columns tested, cholester column is suitable for separation of pigments not only for a broad range of polarity, but also for hydrophobic pigments in a simple mobile phase. This finding can help in the selection of column and HPLC parameters in separating photosynthetic pigments.
Starting Page	202
Ending Page	210
Page Count	9
File Format	PDF HTM / HTML
DOI	10.1016/j.proche.2015.03.029
Volume Number	14
Alternate Webpage(s)	https://core.ac.uk/download/pdf/81981891.pdf
Alternate Webpage(s)	https://doi.org/10.1016/j.proche.2015.03.029
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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