NDLI: Prokaryotic Diversity in Aran-Bidgol Salt Lake, the Largest Hypersaline Playa in Iran

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Prokaryotic Diversity in Aran-Bidgol Salt Lake, the Largest Hypersaline Playa in Iran

Content Provider	Semantic Scholar
Author	Makhdoumi-Kakhki, Ali Amoozegar, Mohammad Ali Kazemi, Bahram Pašić, Lejla Ventosa, Antonio
Copyright Year	2012
Abstract	Prokaryotic diversity in Aran-Bidgol salt lake, a thalasohaline lake in Iran, was studied by fluorescence in situ hybridization (FISH), cultivation techniques, denaturing gradient gel electrophoresis (DGGE) of PCR-amplified fragments of 16S rRNA genes and 16S rRNA gene clone library analysis. Viable counts obtained (2.5-4 × 10(6) cells mL(-1)) were similar to total cell abundance in the lake determined by DAPI direct count (3-4×10(7) cells mL(-1)). The proportion of Bacteria to Archaea in the community detectable by FISH was unexpectedly high and ranged between 1:3 and 1:2. We analyzed 101 archaeal isolates and found that most belonged to the genera Halorubrum (55%) and Haloarcula (18%). Eleven bacterial isolates obtained in pure culture were affiliated with the genera Salinibacter (18.7%), Salicola (18.7%) and Rhodovibrio (35.3%). Analysis of inserts of 100 clones from the eight 16S rRNA clone libraries constructed revealed 37 OTUs. The majority (63%) of these sequences were not related to any previously identified taxa. Within this sampling effort we most frequently retrieved phylotypes related to Halorhabdus (16% of archaeal sequences obtained) and Salinibacter (36% of bacterial sequences obtained). Other prokaryotic groups that were abundant included representatives of Haloquadratum, the anaerobic genera Halanaerobium and Halocella, purple sulfur bacteria of the genus Halorhodospira and Cyanobacteria.
Starting Page	87
Ending Page	93
Page Count	7
File Format	PDF HTM / HTML
PubMed reference number	22185719
Journal	Medline
Volume Number	27
Alternate Webpage(s)	https://www.jstage.jst.go.jp/article/jsme2/27/1/27_ME11267/_pdf
Alternate Webpage(s)	https://doi.org/10.1264/jsme2.me11267
Journal	Microbes and environments
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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