NDLI: Regulation of malate oxidation in plant mitochondria. Response to rotenone and exogenous NAD+.

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Regulation of malate oxidation in plant mitochondria. Response to rotenone and exogenous NAD+.

Content Provider	Semantic Scholar
Author	Palmer, Joseph Michael Schwitzguébel, J. P. Møller, Ian Max
Copyright Year	1982
Abstract	Exogenous NAD+ stimulated the rotenone-resistant oxidation of all the NAD+-linked tricarboxylic acid-cycle substrates in mitochondria from Jerusalem artichoke (Helianthus tuberosus L.) tubers. The stimulation was not removed by the addition of EGTA, which is known to inhibit the oxidation of exogenous NADH. It is therefore concluded that added NAD+ gains access to the matrix space and stimulates oxidation by the rotenone-resistant NADH dehydrogenase located on the matrix surface of the inner membrane. Added NAD+ stimulated the activity of malic enzyme and displaced the equilibrium of malate dehydrogenase; both observations are consistent with entry of NAD+ into the matrix space. Analysis of products of malate oxidation showed that rotenone-resistant oxygen uptake only occurred when the concentration of oxaloacetate was low and that of NADH was high. Thus it is proposed that the concentration of NADH regulates the activity of the two internal NADH dehydrogenases. Evidence is presented to suggest that the rotenone-resistant NADH dehydrogenase is engaged under conditions of high phosphorylation potential, which restricts electron flux through the rotenone-sensitive dehydrogenase (coupled to ATP synthesis).
Starting Page	1
Ending Page	2
Page Count	2
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.biochemj.org/content/ppbiochemj/208/3/703.full.pdf
PubMed reference number	6819864v1
Volume Number	208
Issue Number	3
Journal	The Biochemical journal
Language	English
Access Restriction	Open
Subject Keyword	Adenosine Triphosphate Egtazic Acid Helianthus NADH Nicotinamide adenine dinucleotide (NAD) Oxaloacetates Oxidoreductase Oxygen Thioctic Acid Tissue membrane Tricarboxylic Acids malate oxidation response to rotenone
Content Type	Text
Resource Type	Article

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