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Specific detection of Shigella sonnei by Enzyme-Linked Aptamer Sedimentation Assay
| Content Provider | Semantic Scholar |
|---|---|
| Author | Masoudipour, Elham Mousavi, Seyed Latif Basiri, Mohsen |
| Copyright Year | 2011 |
| Abstract | Shigella species are able to cause Shigellosis – an enteric infection considered as a global human health threat. Development of potent new anti-Shigella agents for rapid and specific detection and treatment is therefore of great importance. Aptamers, nucleic acid oligomers capable of specific binding to a wide range of non-nucleic acid targets, seem to helpthis purpose. In this study, we selected DNA aptamers that bind to S. sonnei whole cell using a Systematic Evolution of Ligands by Exponential enrichment (SELEX) process. The resulting aptamers exhibited specificity in their ability to bind only to S. sonnei cells. Five unique DNA sequences were isolated from the aptamer cocktail by specifically cloning to S. sonnei, among them ASA4 showed the highest affinity. |
| Starting Page | 11 |
| Ending Page | 25 |
| Page Count | 15 |
| File Format | PDF HTM / HTML |
| Volume Number | 1 |
| Alternate Webpage(s) | https://pbiosci.ut.ac.ir/article_22454_30dfb48dc35a77aadd7e717615f45d74.pdf |
| Alternate Webpage(s) | https://pbiosci.ut.ac.ir/article_22186_0f1fc14375d1d28ff7eee0fbcc0311d8.pdf |
| Alternate Webpage(s) | https://doi.org/10.22059/pbs.2011.22454 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
