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Benzyloxycarbonylphenylalanylcitrulline p-nitroanilide as a substrate for papain and other plant cysteine proteinases.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Gray, Christopher J. Boukouvalas, John Szawelski, Ronald J. Wharton, Christopher W. |
| Copyright Year | 1984 |
| Abstract | After preliminary assays, with papain, bromelain and ficin, on a range of citrulline p-nitroanilides, values of Km and kcat. for the papain-catalysed hydrolysis of three derivatives, N alpha- benzyloxycarbonylcitrulline p-nitroanilide, benzyloxycarbonylphenylalanylcitrulline p-nitroanilide and benzyloxycarbonylglycylphenylalanylcitrulline p-nitroanilide, were obtained. It is concluded that benzyloxycarbonylphenylalanylcitrulline p-nitroanilide is a highly selective substrate for the sensitive detection and assay of the plant cysteine proteinases. |
| File Format | PDF HTM / HTML |
| DOI | 10.1042/bj2190325 |
| Alternate Webpage(s) | http://www.biochemj.org/content/ppbiochemj/219/1/325.full.pdf |
| PubMed reference number | 6721861 |
| Alternate Webpage(s) | https://doi.org/10.1042/bj2190325 |
| Journal | Medline |
| Volume Number | 219 |
| Issue Number | 1 |
| Journal | The Biochemical journal |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |