NDLI: Annexin 5 inhibits thyrotropin releasing hormone (TRH) stimulated prolactin release in the primary culture of rat anterior pituitary cells.

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Annexin 5 inhibits thyrotropin releasing hormone (TRH) stimulated prolactin release in the primary culture of rat anterior pituitary cells.

Content Provider	Semantic Scholar
Author	Kawaminami, Mitsumori Senda, Akio Etoh, Seishi Miyaoka, Hiroko Kurusu, Shiro Hashimoto, Inoru
Copyright Year	2004
Abstract	Annexin 5, a novel calcium-phospholipid binding protein, is thought to be involved in hormone secretion by the anterior pituitary gland. Gonadotropin releasing hormone stimulates annexin 5 synthesis, which, in turn, enhances gonadotoropin secretion. On the other hand, annexin 5 was shown to inhibit prolactin release in vitro. To understand the nature of the opposing effects of annexin 5 on these two major pituitary hormones, the present study examines the inhibitory effect of annexin 5 on prolactin release in relation to thyrotropin stimulating hormone (TRH) using primary cultures of anterior pituitary cells of adult female rats. While recombinant rat annexin 5 was found to have little effect on basal prolactin release, it significantly inhibited TRH-stimulated prolactin release. Addition of specific anti-annexin 5 serum to the culture increased basal prolactin release in a concentration dependent manner, and no further increase in prolactin release was observed following application of TRH in the presence of anti-annexin 5. The enhanced basal prolactin release induced by anti-annexin 5 was reversed by the simultaneous administration of indomethacin, an inhibitor of cyclooxygenase. These results demonstrate that endogenous pituitary annexin 5 exerts an inhibitory effect on prolactin release and suggest that this is attained by suppression of eicosanoid synthesis in vitro.
Starting Page	24
Ending Page	28
Page Count	5
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://www.jstage.jst.go.jp/article/endocrj/51/3/51_3_349/_pdf/-char/en
PubMed reference number	15256781v1
Volume Number	51
Issue Number	3
Journal	Endocrine journal
Language	English
Access Restriction	Open
Subject Keyword	Annexins Calcium Corticotropin-Releasing Hormone Eicosanoids Gonadorelin Gonadotropins In Vitro [Publication Type] Indomethacin Phospholipids Pituitary Hormones, Anterior Posterior Pituitary Hormones Recombinant Thyroid-Stimulating Hormone Recombinants Stimulation (motivation) Thyrotropin
Content Type	Text
Resource Type	Article

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