"Kiss-and-run" glutamate secretion in cultured and freshly isolated rat hippocampal astrocytes.

Content Provider	Semantic Scholar
Author	Chen, X. K. Wang, Liecheng Zhou, Yang Zheng, Liang-Hong Zhou, Zhuan
Copyright Year	2005
Abstract	Under physiological conditions, astrocytes not only passively support and nourish adjacent neurons, but also actively modulate neuronal transmission by releasing "glial transmitters," such as glutamate, ATP, and D-serine. Unlike the case for neurons, the mechanisms by which glia release transmitters are essentially unknown. Here, by using electrochemical amperometry and frequency-modulated single-vesicle imaging, we discovered that hippocampal astrocytes exhibit two modes of exocytosis of glutamate in response to various stimuli. After physiological stimulation, a glial vesicle releases a quantal content that is only 10% of that induced by nonphysiological, mechanical stimulation. The small release event arises from a brief (approximately 2 ms) opening of the fusion pore. We conclude that, after physiological stimulation, astrocytes release glutamate via a vesicular "kiss-and-run" mechanism.
Starting Page	465
Ending Page	468
Page Count	4
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.jneurosci.org/content/jneuro/25/40/9236.full.pdf
Alternate Webpage(s)	http://ir.sibs.ac.cn/bitstream/331001/1880/2/9236.full.pdf
Alternate Webpage(s)	http://www2.neuroscience.umn.edu/eanwebsite/PDF%20GJClub/J%20Neurosci%2025%209236%202005.pdf
PubMed reference number	16207883v1
Volume Number	25
Issue Number	40
Journal	The Journal of neuroscience : the official journal of the Society for Neuroscience
Language	English
Access Restriction	Open
Subject Keyword	Adenosine Triphosphate Exocytosis Glutamic Acid L-Serine Dehydratase Neuroglia Synaptic Transmission Vesicle (morphologic abnormality) glutamate secretion serine, D-
Content Type	Text
Resource Type	Article