NDLI: Gravimetric Analysis of the Efficiency of Hydrocarbon Degrading Soil Bacteria Isolated from Oil Contaminated Site

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Gravimetric Analysis of the Efficiency of Hydrocarbon Degrading Soil Bacteria Isolated from Oil Contaminated Site

Content Provider	Semantic Scholar
Author	Saxena, Sonika Tiwari, Diksha Sharma, Baby Bolia, Priya Arora, Sudipti
Copyright Year	2019
Abstract	In the present study, bacterial strains were isolated from an oil contaminated site at Sitapura industrial area Jaipur. Microorganisms used in present investigation were isolated by selective enrichment technique. MSM broth was used in enrichment technique supplemented with 1% v/v hydrocarbon substrate (petroleum oil). Five microbial strains were isolated and showed biodegradation potential for petroleum oil. The results showed that isolated organisms belongs to Alcaligenes sp., Staphylococcus sp., Micrococcus sp., Bacillus sp. and Pseudomonas sp. Highest amount of biodegradation was noticed with Pseudomonas sp. (14.66%) and lower with staphylococcus sp. (3.6%). Optimized conditions of temperature (50p C), pH (6.0), nitrogen source (beef extract) and carbon source (starch) were noticed for Alcaligenes sp. For Staphylococcus sp., temperature of 37p C, pH of 8, nitrogen source as yeast extract and carbon source as starch was noticed. Similarly for Micrococcus sp., optimum temperature of 37p C, pH of 8.0, tryptone as nitrogen source and starch as carbon source were noticed. For bacillus sp., optimized conditions of 50p C, pH 8, yeast extract (as nitrogen source) and starch (as carbon source) were noticed. For Pseudomonas sp., optimized conditions of temperature were 37p C, pH 8, peptone as nitrogen source and sucrose as carbon source were noticed.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://ecochronicle.org/wp-content/uploads/2019/05/ec-140103.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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