NDLI: A fast response fluorescence probe specific for hypochlorous acid detection and its applications in bioimaging.

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A fast response fluorescence probe specific for hypochlorous acid detection and its applications in bioimaging.

Content Provider	Semantic Scholar
Author	Jia, Hongmin Xia, Shuhe Feng, Huan Meng, Qingtao Duan, Chengchen Zhang, Zhiqiang Zhang, Run
Copyright Year	2018
Abstract	Quantitative detection and visualization of hypochlorous acid (HOCl) in biological samples has emerged as a hot topic in biochemical research because of the important role of this biomolecule in live organisms. In this contribution, a novel 1,8-naphthalimide-based fluorescence probe, DNPH-NA, was designed and synthesized for HOCl detection in aqueous solutions and in biological systems. In the presence of HOCl, significant changes in absorption and fluorescence spectra were observed, which allow for the quantitative detection of HOCl in PBS buffer. The detection limit for HOCl was determined to be 50 nM. In the presence of HOCl, the fluorescence response of DNPH-NA was found to be completed within 2 seconds. The desirable features of DNPH-NA for the detection of HOCl in aqueous solutions, such as its high sensitivity and selectivity, reliability at physiological pH, rapid fluorescence response, and biocompatibility enabled its application in the detection of HOCl in biological samples. Imaging of exogenous HOCl in live HeLa cells and endogenous HOCl generation in live J774A.1 macrophage cells and zebrafish was then successfully performed using DNPH-NA as a fluorescence probe.
Starting Page	2074
Ending Page	2082
Page Count	9
File Format	PDF HTM / HTML
PubMed reference number	29508888
Journal	Medline
Volume Number	16
Issue Number	12
Alternate Webpage(s)	http://www.rsc.org/suppdata/c8/ob/c8ob00036k/c8ob00036k1.pdf
Alternate Webpage(s)	https://doi.org/10.1039/c8ob00036k
Journal	Organic & biomolecular chemistry
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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