NDLI: BRAF mutation, but not RET/PTC rearrangements, is correlated with a lower expression of both thyroperoxidase and sodium iodide symporter genes in papillary thyroid cancer

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BRAF mutation, but not RET/PTC rearrangements, is correlated with a lower expression of both thyroperoxidase and sodium iodide symporter genes in papillary thyroid cancer

Content Provider	Semantic Scholar
Author	Romei, Cristina Ciampi, R. Procesi Faviana, Pinuccia Agate, Laura Molinaro, Eleonora Bottici, Valeria Basolo, Fulvio Miccoli, Paolo Pacini, Furio Pinchera, Aldo Elisei, Rossella
Copyright Year	2008
Abstract	A low sodium iodide symporter (NIS) expression has been shown in papillary thyroid carcinomas (PTCs) harboring the BRAFmutation. In the present study, we analyzed the mRNA expression of thyroid differentiation genes, glucose transporter (GLUT)-1 and GLUT-3, in 78 PTCs according to the presence of BRAF or RET/PTC rearrangements. We found BRAF and RET/PTC rearrangements in 35.8 and 19.4% of PTCs respectively. The mRNA expression of NIS and thyroperoxidase (TPO) genes were significantly lower (P!0.0001 and PZ0.004 respectively) in BRAF-positive PTC with respect to non-mutated samples. In support of this result, immunohistochemistry showed that the percentage of NIS-positive cells was significantly lower (PZ0.005) in BRAF-mutated PTC (mean 53.5%) than in negative cases (mean 72.6%). In contrast, no difference either in NIS or in any other thyroid differentiation genes’ mRNA expression was found in PTC with or without RET/PTC rearrangements. When GLUT-1 and GLUT-3 mRNA expression was considered, no correlation was found either in BRAFnor in RET/PTC-mutated cases. In conclusion, this study confirmed the presence of a genetic alteration of BRAF and/or RET oncogenes in 64% of PTC cases and revealed a significant correlation of BRAF mutation with a lower expression of bothNIS and TPO. This latter finding could indicate that an early dedifferentiation process is present at the molecular level in BRAF-mutated PTC, thus suggesting that the previously demonstrated poor prognostic significance of BRAF mutation could be related to the dedifferentiation process more than to a more advanced stage at diagnosis. Endocrine-Related Cancer (2008) 15 511–520
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://erc.bioscientifica.com/downloadpdf/journals/erc/15/2/511.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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