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TGF-beta 1 gene-activated matrices regulated the osteogenic differentiation of BMSCs
| Content Provider | Semantic Scholar |
|---|---|
| Author | Hai-Tao, Pan Qi-Xin, Zheng Xiao-Dong, Guo |
| Copyright Year | 2007 |
| Abstract | Poly (lactic acid/glycolic acid/asparagic acid-co-polyethylene glycol)(PLGA-[ASP-PEG]) scaffold materials were linked with a novel nonviral vector (K)16GRGDSPC through cross linker Sulfo-LC-SPDP to construct a new type of nonviral gene transfer system. Eukaryotic expressing vector containing transforming growth factor beta 1 (pcDNA3-TGFβ1) was encapsulated by the system. Bone marrow stromal cells (BMSCs) obtained from rabbit were cultured on PLGA-[ASP-PEG] modified by (K)16GRGDSPC and TGF-β1 gene and PLGA-[ASP-PEG] modified by (K)16GRGDSPC and empty vector pcDNA3 as control. The expressions of osteogenic makers of the BMSCs cultured on the TGF-β1 gene-activated scaffold materials were found significantly higher than those of the control group (P<0.05). A brand-new way was provided for regulating seed cells to directionally differentiate into osteoblasts for bone defect restoration in bone tissue engineering. |
| Starting Page | 431 |
| Ending Page | 435 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s11595-006-3431-5 |
| Volume Number | 22 |
| Alternate Webpage(s) | https://page-one.springer.com/pdf/preview/10.1007/s11595-006-3431-5 |
| Alternate Webpage(s) | https://doi.org/10.1007/s11595-006-3431-5 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
