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Biofilm Formation in Denture Base Acrylic Resins and Disinfection Method Using Microwave Denture Base Acrylic Resins and Microwave Disinfection
| Content Provider | Semantic Scholar |
|---|---|
| Author | Dantas, Ana Patricia Fernandes Macedo Consani, Rafael Leonardo Xediek Sardi, Janaína De Cássia Orlandi Mesquita, Marcelo Ferraz Silva, Vasconcellos Dos Santos Da Sinhoreti, Mário Alexandre Coelho |
| Copyright Year | 2014 |
| Abstract | The purpose of this study was to verify the effect of the microwave disinfection in denture base acrylic resins with Candida sp biofilm formation. Samples (n=90) of each resin were submitted to ethylene oxide sterilization, and then infected by inoculum of yeast (C. albicans, C. tropicalis and C. dubliniensis) with 200 μL of cultures adjusted to 108 CFU/mL, placed in specific media and aerobically incubated at 37°C for 24 hours for biofilm formation. For each resin and microorganism, samples were irradiated by microwave energy at 650 W for 3 minutes. Non-irradiated samples and without microorganisms samples were considered as control. The samples were washed with saline solution and plated in Sabouraud dextrose agar (SDA) for analysis of the number of colony forming units (CFU/mL). Data were submitted to Kruskal-Wallis followed by Student-NewmanKeuls test (α=0.05). The results demonstrated a significant decrease in the number of viable colonies for all biofilms formed by Candida species. It was demonstrated an effective disinfection of the samples contaminated by Candida biofilm when microwave irradiated. This study demonstrated that microwave disinfection was able to significantly decrease the amount of microorganisms from denture base acrylic resins contaminated and therefore could be used as a preventive method of candidiasis caused by dentures. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ibimapublishing.com/articles/DENT/2014/112424/112424-1.pdf |
| Alternate Webpage(s) | https://ibimapublishing.com/articles/DENT/2014/112424/m112424.pdf |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Acid-Base Equilibrium Agar Base excess:SCnc:Pt:BldA:Qn:Calculated Blomia tropicalis Ab.IgE:ACnc:Pt:Ser:Qn CDISC SDTM Microorganism Terminology Candida albicans Candida dubliniensis Candida sp Ab.IgM:ACnc:Pt:Ser:Qn Candidiasis Colony Forming Unit per Milliliter Colony-forming unit Denture Bases Dentures Ethylene Oxide Sterilization Glucose Microbial Biofilms Microwave Plating Resin Saline Solution SalineOS Sterilization for infection control Strand Displacement Amplification acrylic biofilm formation incubated negative regulation of reactive oxygen species biosynthetic process |
| Content Type | Text |
| Resource Type | Article |
