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Gill Mediates Immune Responses after Grass Carp Reovirus Challenge in Grass Carp ( Ctenopharyngodon Idella )
| Content Provider | Semantic Scholar |
|---|---|
| Author | Qing-Mei, Li Li-Jun, Chen You-Liang, Rao Xiaozhe, Fu Jian-Guo, Su |
| Copyright Year | 2014 |
| Abstract | Gill plays an important physical barrier role in defending environmental microbes. How are immune responses to endogenous viruses in gill? In the present study, mRNA expressions of 12 antiviral immune-related genes were examined by quantitative real-time RT-PCR (qRT-PCR) in grass carp (Ctenopharyngodon idella) gill after grass carp reovirus (GCRV) challenge. The relative values of CiTLR3, CiTLR7, CiTLR22, CiRIG-I, CiMDA5, CiLGP2, CiNOD1, CiNOD2 and CiIFN-I were almost up-regulated at 12h, 24h, 48h and 72h. Additionally, the mRNA expression of CiIgM was triggered at 72h. However, relative expressions of CiMyD88 and CiIPS-1 were down-regulated at 6h, and subsequently increased. To further verify the reliability of viral infection, VP4 gene (outer capsid protein of GCRV, segment 6) was checked by RT-PCR amplification. The results indicate that gill serves as an important immune organ, and plays crucial roles in triggering antiviral immune responses in grass carp. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ssswxb.ihb.ac.cn/fileSSSWXB/cms/news/info/upload//67295516-761e-4899-a108-46ba5155517d.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
