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Plant regeneration from semi-mature zygotic embryos of Dalbergia sissoo Roxb.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Chand, Suresh Singh, Ajay Kumar |
| Copyright Year | 2005 |
| Abstract | Plant regeneration from callus cultures derived from semi-mature zygotic embryos of Dalbergia sissoo Roxb. was achieved. Callus formation occurred on Murashige and Skoog (1962) medium supplemented with 2.26-13.57 μM 2,4-D in combination with 0.46 and 1.16 μM Kn. Maximum response for callus formation was 78.3% on MS medium containing 9.04 μM 2,4-D and 1.16 μM Kn. Shoot regeneration occurred when calli clumps were transferred to MS medium enriched with 2.22-13.32 μM BAP and 1.34 μM NAA. Maximum response (45%) for shoot regeneration was achieved when calli clumps were transferred to MS medium supplemented with 8.88 μM BAP and 1.34 μM NAA. Average number of shoots per callus clump was 7.5 after 15 weeks of culture. In vitro regenerated shoots were rooted on ½ MS medium containing 1.23 μM IBA. Rooted plantlets transferred to pots containing autoclaved peat moss, soil, and compost mixture (1:1:1) were successfully established in pots. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://nopr.niscair.res.in/bitstream/123456789/7725/1/IJBT%204(1)%2078-81.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
