NDLI: Comparative effects of BAP and NAA on explant development of micropropagated saffron (Crocus sativus L) corms

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Comparative effects of BAP and NAA on explant development of micropropagated saffron (Crocus sativus L) corms

Content Provider	Semantic Scholar
Author	Sarhan, Atef M. Z. Habib, Afaf M. Refaat, Azza M. Awadalla, Sabri S. A.
Copyright Year	2013
Abstract	In vitro propagation of saffron is considered to be an efficient alternative method for large-scale propagation of pathogen free corms. The shoots initiated from the Upper parts of the corm and Sprout on the corms maintained at room temperature were excised and used as explants. The scales were removed and the explants were washed with running tap water for 30 minutes and surface sterilized with 90% ethanol for 3 minutes. different types of corm explants (Sprout, upper and lower parts of the corm tissue) were cultured on Murashige and Skoog medium to find out the best explant type cultured to induce the highest explant development. Different concentrations of BAP (6-benzylaminopurine) at the rates of 0.0, 2.0, 4.0 and 6.0 mg/L and NAA (naphthalene-acetic acid) at the concentration were tested. Data indicated that Upper parts surpassed Sprout and lower parts corm explants in explant development. Also, lower parts of the corm surpassed both upper parts and Sprout explants in callus initiation. On the other hand, Lower part of the corm surpassed both Upper part of the corm and Sprout explants in callus initiation. Higher BAP concentration (6.0 mg/L) enhanced callus production. However, BAP at the rate of 4.0 mg/L enhanced shoot length, number of shoots and callus initiation. The best root length, number of roots and callus initiation were obtained when 4.0 mg/L NAA was added to the medium. Also, NAA at the rate of 6.0 mg/L enhanced callus production.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.aensiweb.com/old/jasr/jasr/2013/5141-5145.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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