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Callus Induction and Influence of Culture Condition and Culture Medium on Growth of Thai Aromatic Rice, Khao Dawk Mali 105, Cell Culture
| Content Provider | Semantic Scholar |
|---|---|
| Author | Summart, Jaruwan Panichajakul, Sanha Prathepha, Preecha Thanonkeo, Pornthap |
| Copyright Year | 2008 |
| Abstract | The effect of different concentrations of 2,4-dichlorophenoxy acetic acid (2,4-D) (1, 2, 3, 4 and 5 mg l ) on callus induction of Thai aromatic rice seeds variety KDML105 cultivated on MS medium was 1 investigated. The supplementation of medium with 2 mg l 2,4-D was found most efficient for callus induction, 1 provided calli with quite good in texture and friable in nature. The influences of incubation temperatures (25±2°C and 30±2°C), light (dark and light), sucrose concentrations (2, 3 and 4% (w/v)) and culture media (MS, Gamborg-B5 (B5), Linsmair and Skoog (LS) and Chu medium (N6)) on growth of rice callus were investigated. Although sucrose at 4% and the N6 medium gave the highest growth of callus but the high quality of callus was obtained when it was grown on the MS medium containing 3% sucrose at 25°C under dark condition. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.idosi.org/wasj/wasj5(2)/18.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
