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Effect of Trypan Blue on the Survival of Cultured Trabecular Meshwork Cells
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kang, Hye Ri Hwang, Seok Jin |
| Copyright Year | 2017 |
| Abstract | Purpose: To evaluate the effects of trypan blue (TB) on the survival of cultured human trabecular meshwork cells (HTMCs). Methods: Primarily cultured HTMCs were exposed to 0.05, 0.10 or 0.50% TB for 1, 5 or 30 min. Cellular survival was assessed using the MTT assay and degree of apoptosis was analyzed with flow cytometry using annexin-V/propidium iodide double staining. Results: Long-term exposure or high concentration of TB decreased the survival of HTMCs (p < 0.05). In flow cytometric analysis, exposure to 0.50% TB for 30 min increased the degree of apoptosis (p < 0.05). Commercial TB decreased cell survival after exposure for 5 min and increased the degree of apoptosis after exposure for 30 min (p < 0.05). Conclusions: TB may cause cellular damage of cultured HTMCs and apoptosis could be the underlying mechanism. In TB-assisted cataract surgery, TB should be used for the shortest time possible and removed completely. J Korean Ophthalmol Soc 2017;58(3):327-332 |
| Starting Page | 327 |
| Ending Page | 332 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| DOI | 10.3341/jkos.2017.58.3.327 |
| Volume Number | 58 |
| Alternate Webpage(s) | https://synapse.koreamed.org/Synapse/Data/PDFData/0035JKOS/jkos-58-327.pdf |
| Alternate Webpage(s) | https://doi.org/10.3341/jkos.2017.58.3.327 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
