Loading...
Please wait, while we are loading the content...
Similar Documents
Epsilon-toxin plasmids of Clostridium perfringens type D are conjugative.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Hughes, Meredith L. Poon, Rachael Adams, Vicki Sayeed, Sameera Saputo, Juliann Uzal, Francisco A. McClane, Bruce A. Rood, Julian I. |
| Copyright Year | 2007 |
| Abstract | Isolates of Clostridium perfringens type D produce the potent epsilon-toxin (a CDC/U.S. Department of Agriculture overlap class B select agent) and are responsible for several economically significant enterotoxemias of domestic livestock. It is well established that the epsilon-toxin structural gene, etx, occurs on large plasmids. We show here that at least two of these plasmids are conjugative. The etx gene on these plasmids was insertionally inactivated using a chloramphenicol resistance cassette to phenotypically tag the plasmid. High-frequency conjugative transfer of the tagged plasmids into the C. perfringens type A strain JIR325 was demonstrated, and the resultant transconjugants were shown to act as donors in subsequent mating experiments. We also demonstrated the transfer of "unmarked" native epsilon-toxin plasmids into strain JIR325 by exploiting the high transfer frequency. The transconjugants isolated in these experiments expressed functional epsilon-toxin since their supernatants had cytopathic effects on MDCK cells and were toxic in mice. Using the widely accepted multiplex PCR approach for toxin genotyping, these type A-derived transconjugants were genotypically type D. These findings have significant implications for the C. perfringens typing system since it is based on the toxin profile of each strain. Our study demonstrated the fluid nature of the toxinotypes and their dependence upon the presence or absence of toxin plasmids, some of which have for the first time been shown to be conjugative. |
| Starting Page | 34 |
| Ending Page | 43 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://jb.asm.org/content/189/21/7531.full.pdf |
| PubMed reference number | 17720791v1 |
| Volume Number | 189 |
| Issue Number | 21 |
| Journal | Journal of bacteriology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Arthrogryposis Chloramphenicol Resistance Clostridium perfringens enterotoxin:PrThr:Pt:Body fld:Ord Clostridium perfringens enterotoxin:Prid:Pt:XXX:Nom Enterotoxemia Genotype determination Plasmids Tracer |
| Content Type | Text |
| Resource Type | Article |
