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AHCYL2 (long-IRBIT) as a potential regulator of the electrogenic Na(+)-HCO3(-) cotransporter NBCe1-B.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Yamaguchi, Soichiro Ishikawa, Toru |
| Copyright Year | 2014 |
| Abstract | Although AHCYL2 (long-IRBIT) is highly homologous to IRBIT, which regulates ion-transporting proteins including the electrogenic Na(+)-HCO3(-) cotransporter NBCe1-B, its functions are poorly understood. Here, we found that AHCYL2 interacts with NBCe1-B in bovine parotid acinar cells using yeast two-hybrid, immunofluorescence confocal microscopy and co-immunoprecipitation analyses. Whole-cell patch-clamp experiments revealed that co-expression of AHCYL2 reduces the apparent affinity for intracellular Mg(2+) in inhibition of NBCe1-B currents specifically in a HCO3(-)-deficient cellular condition. Our data unveil AHCYL2 as a potential regulator of NBCe1-B in mammalian cells. We propose that cytosolic ionic condition appropriate for AHCYL2 to function might be different from IRBIT. |
| File Format | PDF HTM / HTML |
| PubMed reference number | 24472682 |
| Journal | Medline |
| Volume Number | 588 |
| Issue Number | 5 |
| Alternate Webpage(s) | https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/55272/2/Supplemental%20Materials%20and%20Methods%20(Yamaguchi%20and%20Ishikawa).pdf |
| Alternate Webpage(s) | https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/55272/1/Supplemental%20Figures%20(Yamaguchi%20and%20Ishikawa).pdf |
| Alternate Webpage(s) | https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/55272/3/Manuscript%20(Yamaguchi%20and%20Ishikawa).pdf |
| Alternate Webpage(s) | https://doi.org/10.1016/j.febslet.2013.12.036 |
| Journal | FEBS letters |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
