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Adverse alterations in mitochondrial function contribute to type 2 diabetes mellitus-related endothelial dysfunction in humans.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kizhakekuttu, Tinoy J. Wang, Jingli Dharmashankar, Kodlipet C. Ying, Rong Rong Gutterman, David D. Speliotes, Elizabeth K. Widlansky, Michael E. |
| Copyright Year | 2012 |
| Abstract | OBJECTIVE Mitochondrial dysfunction plays a key pathophysiological role in type 2 diabetes mellitus (T2DM). Data delineating relationships between mitochondrial and endothelial dysfunction in humans with T2DM are lacking. METHODS AND RESULTS In 122 human subjects (60 with T2DM, 62 without T2DM), we measured endothelial function by brachial artery ultrasound (flow mediated dilation) and digital pulse amplitude tonometery. Endothelial function in arterioles isolated from gluteal subcutaneous adipose was measured by videomicroscopy. In arterioles and mononuclear cells, we measured inner mitochondrial membrane potential (Δψ(m)), mitochondrial mass, and mitochondrial superoxide production using fluorophores. Endothelial function was impaired in T2DM subjects versus control subjects. Δψ(m) magnitude was larger and mitochondrial mass was lower in arterioles and mononuclear cells in T2DM. Mononuclear mitochondrial mass correlated with flow-mediated dilation and pulse amplitude tonometery (ρ=0.38 and 0.33, P=0.001 and 0.02, respectively), and mononuclear mitochondrial superoxide production inversely correlated with flow-mediated dilation (ρ=-0.58, P=0.03). Low doses of 2 different mitochondrial uncoupling agents (carbonyl cyanide m-chlorophenyl hydrazone and 2,4-dinitrophenol) that reduce Δψ(m) magnitude and a mitochondrial-targeted antioxidant (MitoTEMPOL) improved endothelial function and reduced mitochondrial superoxide levels in T2DM arterioles. CONCLUSIONS Mitochondrial dysfunction may play a central role in the impairment of endothelial dysfunction in T2DM. |
| Starting Page | S97 |
| Ending Page | S101 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://atvb.ahajournals.org/content/atvbaha/32/10/2531.full.pdf?download=true |
| Alternate Webpage(s) | http://atvb.ahajournals.org/content/atvbaha/32/10/2531.full.pdf?cited-by=yes&legid=atvbaha%3B32/10/2531 |
| Alternate Webpage(s) | http://atvb.ahajournals.org/content/atvbaha/early/2012/08/09/ATVBAHA.112.256024.full.pdf?download=true |
| Alternate Webpage(s) | http://atvb.ahajournals.org/content/atvbaha/early/2012/08/09/ATVBAHA.112.256024.full.pdf |
| PubMed reference number | 22879582v1 |
| Alternate Webpage(s) | https://doi.org/10.1161/ATVBAHA.112.256024 |
| DOI | 10.1161/atvbaha.112.256024 |
| Journal | Arteriosclerosis, thrombosis, and vascular biology |
| Volume Number | 32 |
| Issue Number | 10 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | 2,4-Dinitrophenol Carbonyl Cyanide m-Chlorophenyl Hydrazone Cyanides Diabetes Mellitus Diabetes Mellitus, Non-Insulin-Dependent Endothelium Hydralazine Hydrazones Inner mitochondrial membrane Large Membrane Potentials Microscopy, Video Mitochondrial Diseases Mitochondrial Inheritance Pathological Dilatation Structure of arteriole Structure of brachial artery Superoxides Uncoupling Agents endothelial dysfunction mesoxalonitrile |
| Content Type | Text |
| Resource Type | Article |
