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Genetic expression of pobA and fabHB in Bacillus licheniformis M 2-7 in the presence of benzo [ a ] pyrene
| Content Provider | Semantic Scholar |
|---|---|
| Author | Rojas-Aparicio, Augusto Hernandez-Eligio, Jose Alberto Toribio-Jiménez, Jeiry Miguel Castellanos-Escamilla, Mildred Romero, Yanet Ramírez |
| Copyright Year | 2018 |
| Abstract | Bacillus licheniformis M2-7 is a heat-resistant bacterium able to biotransform polycyclic aromatic hydrocarbons. It can transform a wide range of these compounds as naphthalene, phenanthrene, pyrene and benzo[a]pyrene. Benzo[a]pyrene is a polycyclic aromatic hydrocarbon of high molecular weight considered as potentially toxic and carcinogenic for humans. Aiming to discover the genes involved in the biotransformation of benzo[a]pyrene, we made a B. licheniformis M2-7 genomic library in E. coli. We isolated two E. coli strains that were able to grow in minimal salt medium supplemented with benzo[a]pyrene. From the analysis of the DNA fragments in the clones H23 and H38, we identified open reading frames coding for 5 possible genes, among them pobA and fabHB, which products are the enzymes 4hydroxybenzoate 3-monooxygenase and the ketoacyl-ACP synthase Rojas-Aparicio, et al. 2 Genetics and Molecular Research 17 (2): gmr16039916 III, respectively. To evaluate the role of these genes in the metabolism of benzo[a]pyrene in B. licheniformis M2-7, we estimated their relative expression through reverse transcription quantitative PCR. Finally, we observed that the genes pobA and fabHB were overexpressed after 3 h under induction with benzo[a]pyrene, suggesting that this strain could use these genes during the metabolism of this PAH, plus it does it in a faster time than that reported for other bacterial genera |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.geneticsmr.org/articles/genetic-expression-of-poba-and-fabhb-in-bacillus-licheniformis-m27-in-the-presence-of-benzoapyrene.pdf |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | AROMATICS Agarose Gel Appendix Aromatic-L-Amino-Acid Decarboxylases Aspidosperma triternatum B anthracis XXX Ql Cult Benzo(a)pyrene Benzocaine 0.2 MG/MG Oral Gel [Benzo-Jel] Bleomycin/Doxorubicin/Prednisone regimen (BAP) Cardiomyopathies Frame (physical object) Genera Hydrocarbons Mathematical induction Metabolic Biotransformation Mixed Function Oxygenases Molecular Weight Naphthalenes Nucleotide Exchange Factor SIL1 Open Reading Frames Open reading frame Polycyclic Hydrocarbons, Aromatic Reading Frames (Nucleotide Sequence) Reverse Transcriptase Polymerase Chain Reaction Reverse Transcription S1PR2 gene Sol-gel Transcription (software) Travis CI Tritium Volt phenanthrene phthalic acid pyrene |
| Content Type | Text |
| Resource Type | Article |
