NDLI: Ribosomal DNA Intergenic Spacer of the Swimming Crab, Charybdis japonica

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Ribosomal DNA Intergenic Spacer of the Swimming Crab, Charybdis japonica

Content Provider	Semantic Scholar
Author	Ryu, Seong Ho Do, Yoon Kyung Hwang, Ui Wook Choe, Chong Pyo
Copyright Year	1999
Abstract	Abstract. We have determined the full sequence of the ribosomal DNA intergenic spacer (IGS) of the swimming crab, Charybdis japonica, by long PCR for the first time in crustacean decapods. The IGS is 5376 bp long and contains two nonrepetitive regions separated by one long repetitive region, which is composed mainly of four subrepeats (subrepeats I, II, III, and IV). Subrepeat I contains nine copies of a 60-bp repeat unit, in which two similar repeat types (60 bp-a and 60 bp-b) occur alternatively. Subrepeat II consists of nine successive repeat units with a consensus sequence length of 142 bp. Subrepeat III consists of seven copies of another 60-bp repeat unit (60 bp-c) whose sequence is complementary to that of subrepeat I. Immediately downstream of subrepeat III is subrepeat IV, consisting of three copies of a 391-bp repeat unit. Based on comparative analysis among the subrepeats and repeat units, a possible evolutionary process responsible for the formation of the repetitive region is inferred, which involves the duplication of a 60-bp subrepeat unit (60 bp-c) as a prototype.
Starting Page	806
Ending Page	809
Page Count	4
File Format	PDF HTM / HTML
PubMed reference number	10594182
Journal	Medline
Volume Number	49
Alternate Webpage(s)	https://page-one.springer.com/pdf/preview/10.1007/PL00006603
Alternate Webpage(s)	https://doi.org/10.1007/PL00006603
Journal	Journal of Molecular Evolution
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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