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Oxidative modification of creatine kinase BB in Alzheimer's disease brain.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Aksenov, Michael Y. Aksenova, Marina V. Butterfield, David Markesbery, William R. |
| Copyright Year | 2000 |
| Abstract | Creatine kinase (CK) BB, a member of the CK gene family, is a predominantly cytosolic CK isoform in the brain and plays a key role in regulation of the ATP level in neural cells. CK BB levels are reduced in brain regions affected by neurodegeneration in Alzheimer's disease (AD), Pick's disease, and Lewy body dementia, and this reduction is not a result of decreased mRNA levels. This study demonstrates that posttranslational modification of CK BB plays a role in the decrease of CK activity in AD brain. The specific CK BB activity and protein carbonyl content were determined in brain extracts of six AD and six age-matched control subjects. CK BB activity per microgram of immunoreactive CK BB protein was lower in AD than in control brain extracts, indicating the presence of inactive CK BB molecules. The analysis of specific protein carbonyl levels in CK BB, performed by two-dimensional fingerprinting of oxidatively modified proteins, identified CK BB as one of the targets of protein oxidation in the AD brain. The increase of protein carbonyl content in CK BB provides evidence that oxidative posttranslational modification of CK BB plays a role in the loss of CK BB activity in AD. |
| Starting Page | 495 |
| Ending Page | 496 |
| Page Count | 2 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.chem.uky.edu/research/Butterfield/dab_pdfs/dab_pdfs/Aksenov%20et%20al%202000%20J%20Neurochemistry%20%2074%20%202520-2527.pdf |
| Alternate Webpage(s) | http://www.chem.uky.edu/research/butterfield/dab_pdfs/Aksenov%20et%20al%202000%20J%20Neurochemistry%20%2074%20%202520-2527.pdf |
| Alternate Webpage(s) | http://www.chem.uky.edu/research/butterfield/dab_pdfs/dab_pdfs/Aksenov%20et%20al%202000%20J%20Neurochemistry%20%2074%20%202520-2527.pdf |
| PubMed reference number | 10820214v1 |
| Volume Number | 74 |
| Issue Number | 6 |
| Journal | Journal of neurochemistry |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Adenosine Triphosphate Alzheimer's Disease Creatine Kinase MB Isoenzyme Dementia Inactive - Biochemical Activity Level Lewy Body Disease Parkinson Disease Post-Translational Protein Processing nervous system disorder protein oxidation |
| Content Type | Text |
| Resource Type | Article |
