Loading...
Please wait, while we are loading the content...
Similar Documents
Genetic variability in Grapevine virus A from Vitis vinifera L. × Vitis labrusca L. in Sichuan, China
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wang, Jianhui Xi, Dehui Chen, Ke-Ling Li, Hongwen Liu, Xueshi Yuan, Shu Ercisli, Sezai Lin, Honghui |
| Copyright Year | 2012 |
| Abstract | Grapevine virus A (GVA) has a quasispecies nature and is closely associated with the rugose-wood disease complex of Vitis vinifera L. In the different growing regions of table grape in Sichuan, southwest China, the incidence of GVA infection was found to be 16.9% by ELISA detection among 178 grape plants (Vitis vinifera L. × Vitis labrusca L.). The results of restriction fragment length polymorphism (RFLP) studies on the polymerase chain reaction (PCR) products of a total of 139 plasmids, cloned from 15 GVA isolates, suggested that the GVA isolates contained highly divergent variants. The plasmids from each GVA isolate that yielded different PCR-RFLP profiles were preferentially chosen for sequencing and were designated as variants. Furthermore, a phylogenetic study based on the analysis of the GVA coat protein genes and RNA silencing suppressor genes showed that the 40 variants obtained from the 15 GVA isolates represented 4 clades, designated as molecular groups I, II, III, and IV, respectively. The variant-specific PCR detection results indicated that the parts of the tested grape plants were specifically infected by mild variants. |
| Starting Page | 542 |
| Ending Page | 551 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| Volume Number | 36 |
| Alternate Webpage(s) | http://journals.tubitak.gov.tr/biology/issues/biy-12-36-5/biy-36-5-7-1203-23.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
