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Hydrocortisone inhibits cellular proliferation by downregulating hepatocyte growth factor synthesis in human osteoblasts.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Tsunashima, Yoshihiko Kondo, Ayami Matsuda, Tomohiro Togari, Akifumi |
| Copyright Year | 2011 |
| Abstract | Glucocorticoids have multiple systemic effects that may influence bone metabolism but also directly affect osteoblasts by decreasing their proliferation. Using human osteoblastic SaM-1 cells, we examined whether the effects of hydrocortisone on cellular proliferation are mediated by hepatocyte growth factor (HGF). Human osteoblasts constitutively express both HGF and c-Met, its receptor. Hydrocortisone decreased the gene and protein expression of HGF as well as proliferation in SaM-1 cells. These hydrocortisone (0.01-1 µM)-induced decreases in HGF synthesis and cellular proliferation occurred in a concentration-dependent manner. However, no hydrocortisone (0.01-1 µM)-induced decrease in cellular proliferation was observed in human osteosarcoma-derived cells (HOS and SaOS-2), which are not able to produce HGF. In the cellular proliferation in SaM-1 cells, the decrease was blocked concentration-dependently by exogenously applied HGF (0.01-3 ng/ml). Furthermore, SU11274 (1 µM), a highly specific inhibitor of c-Met, suppressed the proliferation of SaM-1 cells, but not HOS cells. From these results, we concluded that hydrocortisone inhibits the proliferation of SaM-1 cells by interrupting the autocrine/paracrine loop via the downregulation of HGF synthesis. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.jstage.jst.go.jp/article/bpb/34/5/34_5_700/_pdf |
| PubMed reference number | 21532160v1 |
| Volume Number | 34 |
| Issue Number | 5 |
| Journal | Biological & pharmaceutical bulletin |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Bone Cysts Cell Proliferation Down-Regulation Glucocorticoids Hepatocyte Growth Factor Hydrocortisone Hyperplasia Microgram per Liter Osteoblasts autocrine |
| Content Type | Text |
| Resource Type | Article |
