NDLI: Real-time quantitative PCR.

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Real-time quantitative PCR.

Content Provider	Semantic Scholar
Copyright Year	2001
Abstract	Add: Floor 2,Building A,328#, Wuhe Road,Minhang District,Shanghai,201109,China Tel: +86-21-64954465 Web: www.shinegene.org.cn/english Real Time Quantitative PCR Cat.No.:SG00103 Description Real-time quantitative PCR remains one of the most sensitive and quantitative tools for gene expression used today. Quantitative PCR(qPCR) is a powerful, highly sensitive technique that can be used to quantitate gene expression, determine gene copy number, detect SNPs, and detect DNA from viral and bacterial microorganisms. ShineGene support both SybrGreen and Taqman reaction chemistries. With Taqman chemistry, a labeled probe is included in the reaction to enhance the specificity and sensitivity of target sequence detection. With SyberGreen chemistry, amplified product is detected by its interaction with the SyberGreen fluorescent dye, which selectively binds to double-stranded DNA templates. We also offer primer and probe design services. We manage all aspects of the project from assay design to data analysis. Sample preparation (Nucleic acid isolation, whole genome amplification, etc.) is also available. You can get full service from ShineGene.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.gene-quantification.com/schmittgen-2001.pdf
Alternate Webpage(s)	http://www.gene-quantification.de/schmittgen-2001.pdf
Alternate Webpage(s)	http://gmo-qpcr-analysis.com/schmittgen-2001.pdf
Alternate Webpage(s)	http://www.synthesisgene.com/tools/realtimepcr.pdf
PubMed reference number	11846607v1
Volume Number	25
Issue Number	4
Journal	Methods
Language	English
Access Restriction	Open
Subject Keyword	Clinical Use Template Copy Number DNA, Double-Stranded Fluorescent Dyes Gene Expression Microorganism Quantitative Real-Time Polymerase Chain Reaction Single Nucleotide Polymorphism Tracer Whole Genome Amplification
Content Type	Text
Resource Type	Article

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