NDLI: Bioluminescence screening in vitro (Bio-Siv) assays for high-volume antimycobacterial drug discovery.

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Bioluminescence screening in vitro (Bio-Siv) assays for high-volume antimycobacterial drug discovery.

Content Provider	Semantic Scholar
Author	Resconi, Anna E. Stover, C. Kendall
Copyright Year	1996
Abstract	Bioluminescence-based assays to indicate antimicrobial susceptibility have been developed and validated for recombinant strains of Mycobacterium tuberculosis, Mycobacterium bovis BCG, Mycobacterium avium, and Mycobacterium intracellulare expressing an integrated eukaryotic luciferase gene. MICs determined with these bioluminescence assays for several antimycobacterial agents, including isoniazid, ethambutol, rifampin, amikacin, streptomycin, ciprofloxacin, and clarithromycin, compared favorably with traditional BACTEC methods and visual estimations of the inhibitory end point. Assay methodology has been optimized for the analysis of large numbers of novel compounds and is simple, inexpensive, and labor efficient. The availability of these four recombinant mycobacteria has permitted a strategy for drug discovery employing the nonpathogenic BCG strain for mass screening purposes with subsequent confirmation of activity against the pathogenic mycobacteria. Furthermore, evidence suggests that the BCG-based screen may allow the direct identification of bactericidal agents.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://aac.asm.org/content/40/6/1536.full.pdf
PubMed reference number	8726034v1
Volume Number	40
Issue Number	6
Journal	Antimicrobial agents and chemotherapy
Language	English
Access Restriction	Open
Subject Keyword	Amikacin Bacillus Calmette-Guerin Battey Bacillus Bioluminescence test Ciprofloxacin Clarithromycin Drug Discovery ELECTROSURGICAL DEVICE Ethambutol Genus Mycobacterium Luciferases Mycobacterium avium Mycobacterium bovis Mycobacterium tuberculosis Recombinants Rifampin Streptomycin confirmation - ResponseLevel isoniazid
Content Type	Text
Resource Type	Article

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