NDLI: Producción de los antígenos ESAT-6 y CFP-10 de Mycobacterium tuberculosis y evaluación de sus potencialidades en el serodiagnóstico de la tuberculosis activa en una población cubana

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Producción de los antígenos ESAT-6 y CFP-10 de Mycobacterium tuberculosis y evaluación de sus potencialidades en el serodiagnóstico de la tuberculosis activa en una población cubana

Content Provider	Semantic Scholar
Author	Pimienta-Rodríguez, Elsa T. Rodríguez-Valdés, C. Sarzo-Gómez, Mónica Vallín-Plou, Carlos
Copyright Year	2012
Abstract	Mycobacterium tuberculosis (Mtb) is the etiological agent of human tuberculosis (TB), which globally is the most prevalent disease caused by a single bacterial pathogen. In 2009, worldwide there were an estimated 9,4 million of new cases and it is estimated that one third of the world population is latently infected with Mtb. Due to to this situation, some rapid and accurate diagnostic method is required for controlling the morbility and mortality of this disease. In this work, the genes esat-6 and cfp-10 were cloned in the E. coli plasmid vectors pET3a and pET23d (Promega), respectively. Both proteins were expressed in the soluble fraction, which facilitated their purification by means of the metal-chelate affinity chromatography under native conditions with a purity of 90 - 94 %. By the first time, using the methodology of ELISA, the serodiagnostic potential value of these two proteins was evaluated in a Cuban population. The antibody res- ponse against recombinant CFP-10 and ESAT-6 from the patients group was statistically higher than that from the healthy BCG-vaccinated individuals. The assays showed a specificity of 97,18 % and low sensitivity of 32 %, value that could be affected by the treatment of the patients with anti-TB drugs. This parameter might be increased by means of the inclusion of other immunogenic antigens in the cocktail, considering that antibody responses in TB patients are heterogeneous.
Starting Page	1
Ending Page	8
Page Count	8
File Format	PDF HTM / HTML
Volume Number	43
Alternate Webpage(s)	https://revista.cnic.edu.cu/revistaCB/sites/default/files/articulos/CB-2012-2-076-083.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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