NDLI: Pinocytosis as a select marker of ramified microglia in vivo and in vitro.

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Pinocytosis as a select marker of ramified microglia in vivo and in vitro.

Content Provider	Semantic Scholar
Author	Ranson, P. A. Thomas, W. E. G.
Copyright Year	1991
Abstract	Based on previous observations in tissue culture, we investigated pinocytotic activity as a potential cell marker for brain microglia. This functional activity was assessed in three different preparations derived from rat: primary cultures of mixed cerebral cortical cells, tissue slabs of whole cerebrum, and cultures of isolated or enriched microglial cells. Each preparation was incubated with the fluorescent dye lucifer yellow as a soluble tracer and then processed for light microscopy. Under the conditions utilized, ramified microglia specifically exhibited differentially high pinocytotic labeling in all cases; the dye was mainly localized within the cell somata, where it was sequestered in pinocytotic vesicles. In each preparation, the identity of the labeled cell population was confirmed as microglia through immunohistochemical staining with the monoclonal antibody (MAb) OX-42, a specific microglial marker. Therefore, pinocytotic labeling is proposed as a select cell marker for microglia, which may be extremely useful in the identification and study of ramified microglial cells.
Starting Page	1
Ending Page	47
Page Count	47
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://jhc.sagepub.com/content/39/6/853.full.pdf
PubMed reference number	2033242v1
Volume Number	39
Issue Number	6
Journal	The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Language	English
Access Restriction	Open
Subject Keyword	Fluorescent Dyes In Vitro [Publication Type] Light Microscopy Microglia Monoclonal Antibodies Staining method Tracer Vesicle (morphologic abnormality) incubated tissue culture
Content Type	Text
Resource Type	Article

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