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A Transcription Activator-Like Effector ( TALE ) Toolbox for Genome Engineering
| Content Provider | Semantic Scholar |
|---|---|
| Author | Sanjana, Neville E. Công, Lê Thành Zhou, Yang Cunniff, Margaret M. Feng, Guoping Zhang, Feng |
| Copyright Year | 2013 |
| Abstract | Transcription activator-like effectors (TALEs) are a class of naturally occurring DNA binding proteins found in the plant pathogen Xanthomonas sp. The DNA binding domain of each TALE consists of tandem 34-amino acid repeat modules that can be rearranged according to a simple cipher to target new DNA sequences. Customized TALEs can be used for a wide variety of genome engineering applications, including transcriptional modulation and genome editing. Here we describe a toolbox for rapid construction of custom TALE transcription factors (TALE-TFs) and nucleases (TALENs) using a hierarchical ligation procedure. This toolbox facilitates affordable and rapid construction of custom TALE-TFs and TALENs within one week and can be easily scaled up to construct TALEs for multiple targets in parallel. We also provide details for testing the activity in mammalian cells of custom TALE-TFs and TALENs using, respectively, qRT-PCR and Surveyor nuclease. The TALE toolbox described here will enable a broad range of biological applications. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://fenglaboratory.org//wp-content/uploads/2017/07/a-transcription-activator-like-effector-toolbox-for-genome-engineering-2012.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
