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4-Fluoro-3-nitrophenyl azide binding sites on purified beef liver monoamine oxidase B
| Content Provider | Semantic Scholar |
|---|---|
| Author | Hsu, Mei-Chich Chen, Shiuan Shih, Jean C. |
| Copyright Year | 1987 |
| Abstract | Previous work from this laboratory has shown that 4-fluoro-3-nitrophenyl azide (FNPA) is an effective photoaffinity labeling probe for MAO-B (Chen et al., Biochem. Pharmac.34, 781-785, 1985). The FNPA binding sites have been further studied by using [(3)H]FNPA. When [(3)H]FNPA was photolyzed with purified beef liver MAO, then subjected to tryptic and chymotryptic digestion, three radioactive peaks were observed after Sephadex G-25 column chromatography procedure. The extent of [(3)H]FNPA incorporation varied directly with [(3)H]FNPA concentration. They could be protected by the presence of the substrate (phenylethylamine) or inhibitors (pargyline and trans-phenylcyclopropylamine) of MAO-B during photolysis. These protections were concentration dependent. Furthermore, the decrease in [(3)H]FNPA labeling in the presence of inhibitors paralleled the decrease in MAO catalytic activity. These results suggest that the FNPA binding sites were related to the active site of MAO-B. Under the same conditions, the separation profiles of [(3)H]FNPA labeled and [(3)H]pargyline labeled tryptic-chymotryptic peptides after Sephadex G-25 column chromatography are distinctly different. This result suggests that FNPA labeling sites may be different from the pargyline binding site. Since pargyline binds to the prosthetic group(-FAD) of MAO, [(3)H]FNPA may label different domains of the active site. This probe may be useful for the characterization of the active site of MAO-B. |
| Starting Page | 167 |
| Ending Page | 172 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/0197-0186(87)90123-9 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/0197018687901239 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/0197018687901239?dgcid=api_sd_search-api-endpoint |
| PubMed reference number | 20501066 |
| Alternate Webpage(s) | https://doi.org/10.1016/0197-0186%2887%2990123-9 |
| Journal | Medline |
| Volume Number | 10 |
| Journal | Neurochemistry International |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
